The authors describe the use of Exnase II in restriction enzyme-free cloning of inserts into plasmids. They show that their method leads to a higher frequency of scarless plasmids then the Exnase cloning or Two-step PCR / ligase method. Due to the poor English it took me a while before I was able to understand what the message of the paper was.
with Exnase II (ClonExpress II Vazyme) to generate pLP12‐ ∆pslA and was further confirmed by primers pLP‐UF and pLP‐UR. DH5α and PA47 wild‐type strains were transformed with this plasmid. Deletion of pslA was confirmed by PCR using primers PslA‐MF1 and PslA‐ MR2. Detailed information about the strains and primers is shown in
2021-1-5 · So we need to use Exnase II (C214 Vazyme) to cut the overlapped DNA segment and then recombined to circled DNA S and S-D614G construct 1. Exanse II 0.5ul recombination Exanse II enzyme 1ul 5X buffer 3.5ul DMT-digested product=total 5 ul
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2020-6-1 · The PCR products were ligated into pGEX-4T-1 vector by homologous recombination and exnase II (Vazyme Biotech Nanjing China) and transformed into the Escherichia coli BL21 CodonPlus™ (DE3). The transformant was incubated in Luria-Bertani broth (containing 50 μg/mL ampicillin) at 16–28 °C with agitation of 180 rpm.
2018-7-18 · ClonExpress® II One Step Cloning kit is a new version cloning kit based on ClonExpress® One Step Cloning technology supplied with Exnase® II and reaction buffer. Compared to the previous version Exnase® II is added a unique recombinant enhancer which can significantly improve the efficiency of recombination (100 cfu/ ng Vector) even
ClonExpress® MultiS bufferExnase® MultiS . PCR5 - PCR Exnase® 30 min 95 .
ClonExpress II One-Step Cloning Kit uses a highly efficient homologous recombination technology to clone DNA products to very high efficiency in a simple rapid one-step procedure. Any amplified product(s) can be cloned into to any linearized vector at any site. The kit uses Exnase II a unique recombinant enhancer which can significantly improve the efficiency of recombination (100 cfu/ ng
The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification greatly simplifying The experimental steps.
2015-9-15 · In this study we developed a rapid and efficient Exnase TM II-based in vitro recombination approach for influenza gene cloning into reverse genetics system. And the high efficacy of the strategy was evaluated and confirmed by cloning the eight genes from viruses PR8 and H9N2 into the reverse genetics system. 2.
MedicalExpo C114 Vazyme Biotech Co. Ltd MedicalExpo
2020-11-3 · Phanta max super-fidelity DNA polymerase and Exnase®II were purchased from Vazyme Biotech Co. Ltd. (Nanjing China). Restriction enzymes were purchased from TaKaRa Biotech Co. Ltd. (Tokyo Japan). The wheat bran was procured from a local mill of Jinan city (Shandong China). All other regents were bought from Solarbio Science and Technology
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2020-4-1 · The stir-3 constructs were obtained from SC09 genomic DNA by treatment with recombinase Exnase II (ClonExpress II Vazyme) and cloned into pCMV-Tag 2B vector (Miaoling). 2.10. Co-immunoprecipitation (Co-IP) assay. Rainbow trout head kidney macrophages were infected with wild-type SC09 for 12 h. Cells were washed twice in ice-cold PBS harvested
The plasmids used in the two-hybrid system were constructed using Exnase II (ClonExpress II Vazyme Vazyme). myD88 and Y. ruckeri SC09 stir-2 were amplified from the pCMV-HA-MyD88 vector and SC09 genomic DNA respectively by PCR using Exnase II primers. PCR products were cloned into the pGBKT7 vector downstream of the Gal4 DNA-binding domain
2021-3-15 · VazymeBiotechCo. Ltd Order global vazyme m Support global vazyme m ww w.vazyme m Forresearchuseon ly notforuseindiagnosticprocedures. M DL5 000Marker.
2021-1-5 · So we need to use Exnase II (C214 Vazyme) to cut the overlapped DNA segment and then recombined to circled DNA S and S-D614G construct 1. Exanse II 0.5ul recombination Exanse II enzyme 1ul 5X buffer 3.5ul DMT-digested product=total 5 ul
2020-1-22 · linearized pET28a using Exnase II (Vazyme Co. Nanjing). The re-combinant plasmids were transferred into E. coli BL21(DE3) and ver-ified by colony PCR and sequencing. Heterogeneous expression of the reductases were performed by inoculated the recombinant E. coli BL21(DE3) in LB medium with 50 μg·mL−1 kanamycin and cultured at
2019-3-21 · Ex nase II at37℃for 30 min(F ig.1.). The cloningpro duc tsc anthen be directlytran sfo rmed to com petent cellswith ap ositive rate >95 . ClonExpress MultiS One Step Cloning Kit is anew version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS
The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of cloning and the tolerance to impurities making it possible for linearized vectors and inserts to be directly used for recombination cloning without purification greatly simplifying The experimental steps.
ClonExpress II One Step Cloning Kit C112 Vazyme 1 " >95 "
ClonExpress II One Step Cloning (Vazyme #C112) is a simple fast and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly the vector is
2021-7-20 · ClonExpress ® II Exnase ® II / PCR 5 PCR 5 3 (15
C115-01/02. ClonExpress®UltraOneStepCloningKit 5-15min Mix 50bp 10kb >95 / PCR 5 3 . 800.00.
2021-1-5 · So we need to use Exnase II (C214 Vazyme) to cut the overlapped DNA segment and then recombined to circled DNA S and S-D614G construct 1. Exanse II 0.5ul recombination Exanse II enzyme 1ul 5X buffer 3.5ul DMT-digested product=total 5 ul
ClonExpress MultiS One Step Cloning Kit (Vazyme #C113) is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer in this kit are especially optimized for the assembly of multiple fragments. With the help of this kit up to 5 fragments can be orderly assembled in a single reaction.
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
Mut Express MultiS Fast Mutagenesis Kit V2-Vazyme Biotech Co. Ltd- Mut Express® MultiS Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit three to five discrete sites on the target plasmid can be introduced at one time. The kit consists of two The ultra-high fidelity of Phanta® Max significantly reduces the
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