ClonExpress MultiS One Step Cloning Kit (Vazyme #C113) is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer in this kit are especially optimized for the assembly of multiple fragments. With the help of this kit up to 5 fragments can be orderly assembled in a single reaction.
2021-6-22 · Promoter sequences of PsUFGT (2523bp) and PsGST (2339bp) were amplified and inserted into pGreenII 0800–LUC vector using ClonExpress Ultra One Step Cloning Kit (Vazyme Nanjing China). A fragment of PsMYB10.2 (296–645 bp) was amplified using specific primers (TRV2-PsMYB10.2F and pTRV2-PsMYB10.2R) and inserted into pTRV2 to generate pTRV2
C112-02 ClonExpress® II One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology. Log in . Forgot your password Open account. ClonExpress®Ultra One Step Cloning Kit. 50rxn Wilhelm-Mauser-Str. 41-43 50827 KölnGermany Tel 49 221 9498320
2019-10-15 · VAZYME C115-ClonExpress Ultra One Step Cloning Kit pBluescript II SK( )T7infusion pBluescript II SK( )T7terminator pBluescript II SK( )T7terminatorKpn1Not1 P
2020-7-28 · ClonExpress Ultra One Step Cloning Kit. 515min . Mix . 50 bp 10 kb >95 .
ClonExpress II One Step Cloning Kit ClonExpress Ultra One Step Cloning Kit . 5min Universal Ligation Mix (1 / 1) 1 BioLinks Lab Supplies. Nijenheim 1325. 3704VC Zeist. The Netherlands. Tel 00-31-030-2765796. Email info bio-links. Reviews. Rating 3.6363636363636 stars
2021-4-15 · The pegRNA constructs were made as reported previously 8 by amplifying the spacer-scaffold-RT-PBS fragment by one-step PCR and cloning it into OsU3-sgRNA vectors using a ClonExpress II One Step
C115-02 ClonExpress®Ultra One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology.
2019-8-21 · A ClonExpress Ultra One Step Cloning Kit (Vazyme China) was utilized to introduce mutations in selected AAV1 or AAV5 capsid residues at the
The rabbit HBB gene (GenBank accession number NM_001082260) was cloned into the pCMV-Myc vector (Clontech USA) using the ClonExpress Ultra One Step Cloning Kit (Vazyme China) to obtain the pMyc-HBB plasmid. The pLOV-HBB plasmid encoding HBB protein was obtained by cloning the HBB cDNA sequence into a pLOV-CMV-GFP vector (Neuron Biotech China) using Nhe I and Not I
2020-2-11 · Biolabs (England). ClonExpress II one step cloning kit (Vazyme). DNA used in the experiments was quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific USA). The antibiotics and the concentrations used in culturing B. subtilis were kanamycin (50 μg/ml) chloromycetin (15 μg/ml) ampicillin (100 μg/ml) isopropy-
2020-2-11 · Biolabs (England). ClonExpress II one step cloning kit (Vazyme). DNA used in the experiments was quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific USA). The antibiotics and the concentrations used in culturing B. subtilis were kanamycin (50 μg/ml) chloromycetin (15 μg/ml) ampicillin (100 μg/ml) isopropy-
C115-02 ClonExpress®Ultra One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology.
2020-7-28 · ClonExpress Ultra One Step Cloning Kit. 515min . Mix . 50 bp 10 kb >95 .
2016-8-5 · ClonExpress II One Step Cloning Kit (Vazyme Biotech Co. Ltd). Typically 20 μL of a mixture containing 4 μL of 5 CE II Buffer 2 μL of Exnase II 50 ng linear plasmid 100 ng PCR fragment and ddH2O was incubated at 37oC for 30 min and the resulting solution was used to transform chemically competent E. coli DH5α cells. Colony PCR and
ClonExpress II One Step Cloning Kit-——-ClonExpress®IIOneStepCloningKit 50bp-10kbDNA
2021-5-1 · 2020 Product Catalogue Reagents for Molecular Biology Research Contents Contents Product List 01 Applications 06 PCR 07 Cloning / Mutagenesis 11 Nucleic Acid Electrophoresis 14 Reverse Transcription 15 qPCR 17 Nucleic Acid Isolation 20 Cell Biology / Protein Research 24 Genome Editing 28 Selected Product Citations 29
2021-7-22 · ClonExpress II One Step Cloning Kit C112 Vazyme 6001000 ClonExpress MultiS One Step Cloning Kit Vazyme 5201080
2020-3-2 · One involves direct uptake into nodule epidermal cells involving reduction of ferric to GFP constructs using the ClonExpress II One Step Cloning Kit (Vazyme in 100 ethanol for 20 min. Samples were then embedded in Spurr s resin and sliced into 70 nm thick sections with an ultra-thin microtome (Leica UC7 Leica Microsystems ).
2021-6-22 · Promoter sequences of PsUFGT (2523bp) and PsGST (2339bp) were amplified and inserted into pGreenII 0800–LUC vector using ClonExpress Ultra One Step Cloning Kit (Vazyme Nanjing China). A fragment of PsMYB10.2 (296–645 bp) was amplified using specific primers (TRV2-PsMYB10.2F and pTRV2-PsMYB10.2R) and inserted into pTRV2 to generate pTRV2
2021-6-12 · SmUxe was further introduced into the BamHI site of pCZ192-1 and pCZ192-2 via ClonExpress II One Step Cloning Kit to accomplish pCZ193-1 and pCZ193-2. The cassette of SmUxs1-SmUxe was amplified using pCZ192-1 as template and inserted into the Not I site of pCZ165 (pET28a harboring OsUGT708A40 Additional file 1 Table S1) to accomplish pCZ194
ClonExpress® Ultra One Step Cloning Kit HiScript II Q RT SuperMix for qPCR g DNA wiper -100ml 8/ opc Mix(green)
2019-8-21 · A ClonExpress Ultra One Step Cloning Kit (Vazyme China) was utilized to introduce mutations in selected AAV1 or AAV5 capsid residues at the
2020-7-28 · ClonExpress Ultra One Step Cloning Kit. 515min . Mix . 50 bp 10 kb >95 .
2021-6-22 · Promoter sequences of PsUFGT (2523bp) and PsGST (2339bp) were amplified and inserted into pGreenII 0800–LUC vector using ClonExpress Ultra One Step Cloning Kit (Vazyme Nanjing China). A fragment of PsMYB10.2 (296–645 bp) was amplified using specific primers (TRV2-PsMYB10.2F and pTRV2-PsMYB10.2R) and inserted into pTRV2 to generate pTRV2
2020-4-30 · (China). ClonExpress® II One Step Cloning Kit (Vazyme Biotech Co. Ltd. Nanjing China) was used for plasmid con-struction. The other reagents were obtained from Thermo Fisher Scientific. EliDH5αwascultivatedinLuria–Bertanimedium(LB medium) at 37 °C and 200 rpm for kstD gene cloning. E. coli BL21(DE3
2020-7-28 · ClonExpress Ultra One Step Cloning Kit. 515min . Mix . 50 bp 10 kb >95 .
2021-6-16 · ClonExpress ® Ultra One Step Cloning Kit 1-5 2 ClonExpress Mix 50℃5-15 min . . C115-01 (25 rxn) C115-02 (50 rxn) 2 ClonExpress ® Mix. 125 μl. 2 125 μl. pCE
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